Thermo Fisher Scientific Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme) Monoclonal Antibody (SOD1, 4248)
인간 SOD1 항산화 효소를 표적하는 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 연구 응용에 적합. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. 세포 내 활성산소 제거 관련 연구에 활용 가능.
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Thermo Fisher Scientific Superoxide Dismutase 1 (SOD1) Monoclonal Antibody (SOD1, 4248)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOD1, 4248 |
| Immunogen | Recombinant fragment (around aa14–148) of human SOD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
SOD1 (superoxide dismutase 1) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This soluble cytoplasmic homodimer converts harmful superoxide radicals into molecular oxygen and hydrogen peroxide. The other isozyme is mitochondrial. Mutations in this gene are associated with familial amyotrophic lateral sclerosis (ALS). Rare transcript variants have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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