Takara Cells to cDNA: CellAmp whole transcriptome amplification kit for RT-PCR
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Overview
Allow direct amplification of cDNA from a small number of cultured cells (<1,000) using real-time RT-PCR
Allows real-time PCR (qPCR) analysis of multiple genes from small samples
Expression analysis for low-expressed gene
More Information
Applications
Cells to cDNA with minimal purification
Real-time RT-PCR (RT-qPCR)
cDNA amplification from trace amounts of RNA
| Choosing a PrimeScript kit for real-time RT-PCR | |
|---|---|
| If you want to… | Use this… |
| Make cDNA using a master mix that includes RTase and primers | PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B) |
| Make cDNA using a kit that provides RTase and primers as separate components | PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B) |
| Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR | PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B) |
| Perform 1-step real-time RT-PCR with TB Green | One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B) |
| Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells | CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734) |
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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