Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 800 4X PEG

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:30,000

Immunohistochemistry (IHC)

1:100-1:2,000

Immunocytochemistry (ICC/IF)

1:2,000

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified Rabbit IgG, whole molecule

Conjugate

DyLight™ 800 4X PEG DyLight™ 800 4X PEG DyLight™ 800 4X PEG

View additional formats

• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-5 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-5 nm colloidal gold/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/HRP/host/Goat/)
• [PE-Cyanine5.5](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/PE-Cyanine5.5/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

789/794 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

proprietary buffer

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556775

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product SA5-35571 reacts with the heavy chains of Rabbit IgG and with the light chains common to most Rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol without and store at -20°C. Note: not compatible with sodium azide. Mole Dye/Mole Protein Ratio is lot-dependent.

DyLight 800-4xPEG has an excitation (nm) of 783 nm (Amax) and an emission (nm) of 797 nm (Emax) in PBS. The emission spectra for DyLight™ 800-4xPEG conjugate match the principal output wavelengths of most common fluorescence instrumentation.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.