
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 800 4X PEG
DyLight 800 4X PEG로 표지된 염소 유래 anti-Mouse IgG(H+L) 2차 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 높은 특이성과 신호 증폭 효율 제공. 789/794 nm의 발광 특성으로 근적외선 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:30,000 |
| Immunohistochemistry (IHC) | 1:100–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | DyLight™ 800 4X PEG |
| Excitation/Emission Max | 789/794 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556774 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product SA5-35521 reacts with the heavy chains of Mouse IgG and with the light chains common to most Mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Note: Not compatible with sodium azide.
Mole Dye/Mole Protein Ratio is lot-dependent.
DyLight 800-4xPEG has an excitation (Amax) of 783 nm and an emission (Emax) of 797 nm in PBS.
The emission spectra for DyLight™ 800-4xPEG conjugate match the principal output wavelengths of most common fluorescence instrumentation.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting, or purification of their specified target. Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
- Agarose
- Alexa Fluor 488 / 555 / 647 / 680 / 790
- Alexa Fluor Plus 405 / 488 / 647
- AP, bGal, Biotin
- DyLight 350 / 405 / 488 / 550 / 594 / 633 / 650 / 680
- FITC, HRP, PE, Rhodamine, TRITC
- WesternDot 625
- Custom conjugation available upon request
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