Thermo Fisher Scientific Trypsin Inhibitor Polyclonal Antibody, HRP

Applications

• Western Blot (WB): 1:1,000
• Immunoprecipitation (IP): 1:250

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, as well as purified and partially purified Trypsin Inhibitor [Lima Bean].
Cross-reactivity against Trypsin Inhibitor from other tissues and species may occur but has not been specifically determined.

Target Information

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, and becomes active in the gastrointestinal tract where it degrades proteins to large peptides.
Trypsin prefers lysine and arginine residues. One of its substrates, chymotrypsinogen, is cleaved to produce active chymotrypsin.
High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and are used to screen for and diagnose cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.