Thermo Fisher Scientific RFC4 Polyclonal Antibody
RFC4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 18–323 of RFC4 (Uniprot ID: P35249) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11157207 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver
- Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Human Replication Factor C (RFC) is a hetero-pentameric protein composed of p140, p40, p38, p37, and p36 subunits. RFC functions in loading the DNA polymerase delta processivity factor (PCNA, Proliferating Cell Nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus1, Rad1, and Rad9.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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