Thermo Fisher Scientific PHD1 Polyclonal Antibody
Human EGLN2을 인식하는 Rabbit Polyclonal Antibody로, ICC/IF에 0.25–2 µg/mL 농도로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 및 40% glycerol buffer에 보관. 세포 내 산소 감지 및 HIF 조절 연구에 적합.
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Applications
- Immunocytochemistry (ICC/IF): 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EGLN2. Recombinant protein control fragment (Product #RP-106720). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2791455 |
Product Specific Information
Immunogen sequence:
PLSQALPQLP GSSSEPLEPE PGRARMGVES YLPCPLLPSY HCPGVPSEAS AGSGTPRATA TSTTASPLRD GFGGQDGGE
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 (known as PHD1, PHD2, and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits.
Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis.
The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
HIF PHD1 is widely expressed, with the highest levels in testis, functioning as a cellular oxygen sensor and playing an important role in cell growth regulation.
It can localize to the nucleus or cytoplasm and is detected in hormone-responsive tissues, such as normal and cancerous mammary, ovarian, and prostate epithelium.
HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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