Thermo Fisher Scientific p53 Polyclonal Antibody
인간 p53 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% glycerol, 0.02% sodium azide) 용액 형태로 제공됩니다. 세포 스트레스 반응 연구 및 암 관련 단백질 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TP53. Recombinant protein control fragment (Product #RP-104918) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791210 |
Product Specific Information
Immunogen sequence:
KKGEPHHELP PGSTKRALPN NTSSSPQPKK KPLDGEYFTL QIRGRERFEM FRELNEALEL KDAQAGKEPG GSRAHSSHLK SKKGQSTSR
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. Upon stress, p53 activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.
p53 structure includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 is typically low in normal cells but highly expressed in transformed cells, contributing to malignancy. Mutations in TP53 can abolish DNA binding and tumor suppressor function, observed in many human cancers and genetic syndromes like Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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