Thermo Fisher Scientific Human GAPDH Recombinant Protein

Applications

• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

After reconstitution, store at -80ºC and avoid freeze and thaw cycles.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH.

Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling.

Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion and neuronal apoptosis in cancer.

GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein (mutations of which cause some forms of Alzheimer’s disease) and the polyglutamine tracts of Huntingtin (aberrant forms of which are causative of Huntington’s disease). Associations between GAPDH, actin, and tubulin have also been reported.

Since GAPDH is expressed at high levels in most tissues, it is useful as a protein loading control in Western blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.