Thermo Fisher Scientific KISS1 Receptor (GPR54) (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Flow Cytometry (Flow)

5 µg

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R, 3rd extracellular loop if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KISS1R,uniProtId:Q969F8-1,ncbiNodeId:9606,antigenRange:292-305,antigenLength:398,antigenImageFileName:AKR-001-200UL_KISS1R_Q969F8-1_Rabbit.svg,antigenImageFileNamePDP:AKR-001-200UL_KISS1R_Q969F8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.75 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

GCPR54 is a galanin-like G protein coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the protein suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that it appears to play a role in the onset of puberty. GPR54 has been reported in various human brain regions, placenta, pancreas, and spinal cord, and at lower levels in spleen, peripheral blood leukocytes, testis, lymph node, thymus, small intestine, stomach, lung, and kidney. The receptor is also abundant in tumor tissues. In rat, GPR54 is expressed in liver, intestine, and in the pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex (including frontal), and striatum. ESTs have been isolated from normal placenta and kidney cancer libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.