Thermo Fisher Scientific PODN Polyclonal Antibody
PODN 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human 및 Mouse 시료 반응. Protein A로 정제된 고순도 항체이며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human PODN, amino acids 31–130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Non-collagenous proteins of the small leucine-rich repeat (SLR) protein family are important components of the extracellular matrix. The extracellular matrix regulates cell proliferation, differentiation, adhesion, and migration, playing a key role in development, tissue repair, and metastasis.
Podocan (also known as PCAN, SLRR5A, or PODN) is a 613 amino acid secreted protein belonging to the small leucine-rich proteoglycan (SLRP) family, class V subfamily. It is expressed in kidney, heart, liver, pancreas, and vascular smooth muscle cells. Podocan has three alternatively spliced isoforms containing twenty LRR (leucine-rich repeats), a unique N-terminal cysteine-rich cluster, and a highly acidic C-terminal domain. It is a glycoprotein with N-linked oligosaccharides, potentially involved in growth regulation in cardiovascular tissues. The gene encoding Podocan is located on human chromosome 1 and interacts with type I collagen (COL1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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