Thermo Fisher Scientific Avidin Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Avidin (Hen Egg White).
Cross-reactivity against Avidin from other tissues and species may occur but has not been specifically determined.

Target Information

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag commonly used for immunodetection methods.
This protein has four identical subunits with a combined molecular mass of 67,000–68,000 Da.
Each subunit binds one molecule of biotin, involving tryptophan and lysine residues at the binding site.
The subunit sequence consists of 128 amino acids, with an isoelectric point of 10–10.5.
Avidin is highly soluble in water and salt solutions and stable across a wide pH and temperature range.
Its activity is minimally affected by chemical modification, making it useful for detection and protein purification.
Pure avidin is a key component in certain immunohistochemistry (IHC) methods.
Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.