Thermo Fisher Scientific APC-AAT complexes Monoclonal Antibody (7)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

7

Immunogen

Recombinant form of human activated protein C.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070442

Product Specific Information

ABS 001-07-02 has specificity for a conformation-dependant neoepitope that is expressed in activated protein C upon complex-formation with alpha1-antitrypsin. No reaction is seen to non-complexed alpha1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent.

ABS 001-07-02 has been successfully used in ELISA procedures. ABS 001-07-02 reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note, that the conformational neoepitope expressed in the complex can also be expressed in APC coated directly onto a high-binding microtiter plate.

The epitope specificity of ABS 001-07-02 is not determined.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

The neoepitope is conformation-dependent and expressed in activated protein C upon complex-formation with α1-antitrypsin. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which α1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.