Thermo Fisher Scientific Cyclin B1 Monoclonal Antibody (5G6)
Cyclin B1 단백질을 검출하기 위한 Mouse monoclonal antibody(클론 5G6). Western blot, ICC/IF, Flow cytometry, ELISA에 적합. Human 및 Rat 시료에 반응. 세포 주기 G2/M 전이 조절 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G6 |
| Immunogen | Purified recombinant fragment of human CCNB1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10987087 |
Product Specific Information
MA5-15714 targets CCNB1 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human and Rat samples.
The immunogen is a purified recombinant fragment of human CCNB1 expressed in E. coli.
MA5-15714 detects CCNB1, which has a predicted molecular weight of approximately 60 kDa.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs).
Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. It complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two transcript variants exist: one constitutively expressed and one cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis via APC-dependent mechanisms.
Overexpression of Cyclin B1 has been observed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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