Thermo Fisher Scientific XPF Monoclonal Antibody (OTI3H10), TrueMAB
ERCC4(XPF) 단백질을 인식하는 Mouse monoclonal antibody. Western blot, ICC/IF, Flow cytometry에 적합. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 다양한 종(Dog, Human, Mouse, Rat)에 반응하며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H10 |
| Immunogen | Full length human recombinant protein of human ERCC4 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
When using this carrier-free antibody for conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The structure-specific ERCC1/XPF endonuclease complex is involved in the repair of two distinct types of DNA lesions:
- NER (Nucleotide Excision Repair) for UV-induced lesions and bulky chemical adducts
- Recombination repair of genotoxic interstrand cross-links
In mammalian cells, XPG cleaves the 3′ side of the DNA lesion, while the ERCC1/XPF complex makes the 5′ incision.
ERCC1/XPF likely plays a role in processing partially repaired recombination intermediates.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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