Thermo Fisher Scientific TET2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

ChIP assay (ChIP)

5 µg

-

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 429 and 637 of TET2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.88 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient

RRID

AB_2553157

Product Specific Information

Recommended positive controls: TET2-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2`s role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.