Thermo Fisher Scientific IKB alpha Polyclonal Antibody
IKB alpha 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ELISA, IP에 활용 가능. 인간, 마우스, 랫트 반응성. 고농도(80 mg/mL) 액상 형태로 제공되며 -20°C에서 안정적 보관 가능.
- 카탈로그번호
- 100-4167C
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific IKB alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IKB alpha antibody was produced by repeated immunizations with a synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 80 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Note: Anti-IkBa may react non-specifically with other proteins. Control peptide (code #100-4167P) competes only with the specific reaction of antiserum with IkBa.
Target Information
IkB-alpha is a 40 kDa protein that inhibits NF-kappaB activity through protein-protein interactions in the cytosol. This interaction masks the nuclear localization sequence of NF-kappaB, preventing its translocation to the nucleus. Various stimuli such as TNF-alpha, IL-1 beta, PMA, LPS, UV irradiation, and viral infection can trigger degradation of IkB-alpha, liberating NF-kappaB to activate gene expression.
In humans, the gene is located on chromosome 14q. Activation of NFkB requires phosphorylation of IkB on serines 32 and 36 by IKK alpha (CHUK). IKK alpha, together with IKK beta and IKK gamma, forms the IKK complex essential for NFkB activation in response to proinflammatory cytokines.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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