Thermo Fisher Scientific ANK1 Monoclonal Antibody (3C3)

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.3 ng/mL

-

Immunoprecipitation (IP)

1-5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3C3

Immunogen

ANK1 (AAH30957, 1 a.a. approximately 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ankyrin 1,uniProtId:P16157-1,ncbiNodeId:9606,antigenRange:1-1881,antigenLength:1881,antigenImageFileName:H00000286-M01_Ankyrin_1_P16157-1_House_mouse.svg,antigenImageFileNamePDP:H00000286-M01_Ankyrin_1_P16157-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MWTFVTQLLV TLVLLSFFLV SCQNVMHIVR GSLCFVLKHI HQELDKELGE SEDLSDDEET ISTRVVRRRV FLKGNEFQNI PGEQVTEEQF TDEQGNIVTK KIIRKVVRQI DLSSADAAQE HEEVELRGSG LQPDLIEGRK GAQIVKRASL KRGKQ

Target Information

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.