Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), PE-Cyanine7, eBioscience

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Not specified
• View 2 publications

Flow Cytometry (Flow)

• Recommended dilution: 5 µL (1 µg)/test
• View 9 publications

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 650 / 786
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 660-120S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 590 / 660 / 755
• PE
• PE-Cyanine5
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation

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Product Specific Information

Description:
The W6/32 monoclonal antibody reacts with human MHC class I antigens (HLA-A, B, C). These antigens, associated with beta 2-microglobulin, are expressed by all nucleated human cells and are essential for cell-mediated immune response and tumor surveillance. The W6/32 clone recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates 43 kDa and 11–12 kDa chains. Cross-reactivity has been observed in baboon, rhesus, and cynomolgus monkey.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested by flow cytometry using normal human peripheral blood cells
• Recommended use: 5 µL (1 µg) per test (defined as 100 µL final volume; cell number 10⁵–10⁸/test)

Light Sensitivity:

• PE-Cyanine7 tandem dye is sensitive to photo-induced oxidation; protect from light

Fixation:

• Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency

Excitation / Emission:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:

• 0.2 µm post-manufacturing filtered

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Target Information

HLA-class I MHC antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with beta2 microglobulin. These molecules present foreign peptides for immune recognition by cytotoxic T cells, playing a crucial role in antiviral and antitumor immunity. HLA-class I includes HLA-A, HLA-B, and HLA-C molecules.

HLA and MHC antibodies are essential tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens via high-performance liquid chromatography coupled with tandem mass spectrometry.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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