Thermo Fisher Scientific EEF1G Polyclonal Antibody
EEF1G 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 실험에 적합합니다. Human, Mouse, Rat에 반응하며, 액상 형태로 제공됩니다. 친화 크로마토그래피로 정제되었으며, 4°C 단기 또는 -20°C 장기 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,800 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within human EEF1G aa 10–60/437 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913472 |
Product Specific Information
- Positive Control: Daudi cell lysate, NIH/3T3 cell lysate, mouse cerebellum tissue, human esophagus tissue, rat pancreas tissue
- Subcellular Location: Cytosol, extracellular exosome, nucleus, cytoplasm, membrane
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for delivering aminoacyl-tRNAs to the ribosome. EF-1 gamma (EEF1G or GIG35) is a 437 amino acid subunit of this complex. It is expressed in stomach, pancreas, brain, lung, kidney, intestine, liver, and spleen.
EEF1G contains an N-terminal Glutathione transferase domain that may help anchor the complex to cellular structures and plays a role in assembling multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a role in oncogenic transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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