Thermo Fisher Scientific APIP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.04–0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Immunogen sequence:
NTPEEKDLKD RMAHAMNEYP DSCAVLVRRH GVYVWGETWE KAKTMCECYD YLFDIAVSMK KVGLDPSQLP VGENGIV

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified from human and mouse and designated Apaf 1 (apoptosis protease-activating factor 1).
Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), leading to Caspase-9 activation. Activated Caspase-9 cleaves and activates Caspase-3, a key protease responsible for the proteolytic cleavage of many proteins in apoptosis.

Cho et al. identified a new Apaf 1 Interacting Protein (APIP, also known as CG129 and MMRP19), which acts as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1.
APIP is predicted to code for a 204 amino acid protein. An isoform, APIP2, encodes a 242 amino acid protein differing in its N-terminus.
APIP transcript is ubiquitously expressed in most adult tissues, with high expression in skeletal muscle, heart, and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.