Thermo Fisher Scientific nNOS Recombinant Rabbit Monoclonal Antibody (ST520)
nNOS 단백질을 검출하기 위한 재조합 토끼 단클론 항체(ST520). Western blot, IHC, ICC, Flow cytometry에 적합. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST520 |
| Immunogen | Synthetic peptide within Human nNOS aa 1398–1434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2809460 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Nitric oxide synthase (NOS) activity requires homodimerization and the presence of three cosubstrates (L-arginine, NADPH, O₂) and five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, heme).
Distinct NOS isoforms include:
- NOS1 (nNOS, ncNOS): neuronal form
- NOS3 (ecNOS): endothelial form
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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