Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (HA58), NovaFluor Yellow 690, eBioscience

Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (HA58), NovaFluor Yellow 690

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test
Publications: [References not provided]

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90–110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, and endothelial cells. Expression of CD54 increases upon lymphocyte activation, and interaction with its ligand CD11a plays a key role in inflammation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells
Recommended use ≤ 5 µL (0.4 µg) per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer Usage:

• Add 5 µL per sample (regardless of the number of NovaFluors in the panel).
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).
  CellBlox Plus Blocking Buffer provides improved formulation and is required for optimal staining.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow)

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein composed of five immunoglobulin-like domains, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares homology with ICAM-2 and ICAM-3. ICAM-1 binds integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.

Expression:
Detected on activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B/T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases during inflammation and is also found in certain carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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