Thermo Fisher Scientific Renal Cell Carcinoma (Carbonic Anhydrase IX) Monoclonal Antibody (CA9/4019)
Renal Cell Carcinoma 관련 Carbonic Anhydrase IX 단백질을 인식하는 Mouse monoclonal antibody. Western blot, IHC, Flow Cytometry 등 다양한 응용에 사용 가능. Human 시료 반응성, Protein A/G 정제, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CA9/4019 |
| Immunogen | Recombinant human CA9 protein fragment (around aa 314–410) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Carbonic anhydrase (CA) is an enzyme that facilitates the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions, and is abundant in mammalian tissues.
CA IX is a hypoxia-inducible gene regulated by HIF-1α and is stable within the membrane. Carbonic anhydrases play a key role in pH regulation by controlling hydrogen ion (H⁺) flux.
Under hypoxic conditions, inhibition of CA IX leads to increased cell death, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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