Thermo Fisher Scientific POFUT1 Polyclonal Antibody
POFUT1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 사용 가능. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 완충액에 0.02% sodium azide 포함, 2–8°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide from near the amino terminus of human POFUT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage, store at -20°C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153658 |
Product Specific Information
- Suggested positive control: human heart tissue lysate
- PA5-20581 can be used with blocking peptide PEP-0701
Target Information
POFUT1 is an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family. It adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in epidermal growth factor-like repeats of several cell surface and secreted proteins.
Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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