Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Brilliant Ultra Violet 615, eBioscience
OVA257-264(SIINFEKL) 펩타이드가 결합된 H-2Kb 특이 단클론 항체로, Brilliant Ultra Violet 615 형광표지. 마우스 시료의 항원제시세포(APC) 분석에 적합하며, Flow Cytometry용으로 최적화. 광민감성 주의 및 4°C 암소 보관 권장.
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Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody
Clone: eBio25-D1.16 (25-D1.16)
Conjugate: Brilliant Ultra Violet™ 615
Supplier: Thermo Fisher Scientific (eBioscience™)
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 615 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio25-D1.16 (25-D1.16) |
| Conjugate | Brilliant Ultra Violet™ 615 |
| Excitation / Emission Max | 350 / 607 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3074016 |
Additional Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 650
- Brilliant Violet 711
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The 25-D1.16 monoclonal antibody specifically recognizes the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not unbound H-2Kb or irrelevant peptide complexes.
It is useful for tracking the quantity and localization of antigen-presenting cells (APC) in vivo.
Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes; ≤0.5 µg per test (100 µL final volume, cell count 10⁵–10⁸ cells/test).
Careful titration recommended for optimal assay performance.
Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye emitting at 615 nm, excited by a 355 nm UV laser. Ensure cytometer compatibility.
Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) with minimal impact on brightness.
Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet
Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Usage:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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