Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody

Clone: eBio25-D1.16 (25-D1.16)
Conjugate: Brilliant Ultra Violet™ 615
Supplier: Thermo Fisher Scientific (eBioscience™)

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 650
• Brilliant Violet 711
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The 25-D1.16 monoclonal antibody specifically recognizes the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not unbound H-2Kb or irrelevant peptide complexes.
It is useful for tracking the quantity and localization of antigen-presenting cells (APC) in vivo.

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes; ≤0.5 µg per test (100 µL final volume, cell count 10⁵–10⁸ cells/test).
Careful titration recommended for optimal assay performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye emitting at 615 nm, excited by a 355 nm UV laser. Ensure cytometer compatibility.

Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) with minimal impact on brightness.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Usage:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지