Thermo Fisher Scientific H2AFY2 Monoclonal Antibody (OTI1C2), TrueMAB
Human H2AFY2 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(P) 검증 완료. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. Transcription 억제 및 X 염색체 불활성화 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2-261 of human H2AFY2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Variant histone H2A replacing conventional H2A in specific nucleosomes, repressing transcription.
Histones compact DNA into chromatin and regulate DNA accessibility essential for transcription, replication, and repair.
Post-translational modifications and nucleosome remodeling constitute the histone code that controls gene expression.
H2AFY2 may contribute to stable X chromosome inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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