Thermo Fisher Scientific COX2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of COX2/PTGS2 (NP_0009541) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:COX2,uniProtId:P35354-1,ncbiNodeId:9606,antigenRange:375-604,antigenLength:604,antigenImageFileName:PA5-88606_COX2_P35354-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88606_COX2_P35354-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805028

Product Specific Information

Positive Samples: A-549, HeLa, Mouse spleen, Mouse lung, Rat lung, Rat kidney; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein

Immunogen sequence: PLLPDTFQIH DQKYNYQQFI YNNSILLEHG ITQFVESFTR QIAGRVAGGR NVPPAVQKVS QASIDQSRQM KYQSFNEYRK RFMLKPYESF EELTGEKEMS AELEALYGDI DAVELYPALL VEKPRPDAIF GETMVEVGAP FSLKGLMGNV ICSPAYWKPS TFGGEVGFQI INTASIQSLI CNNVKGCPFT SFSVPDPELI KTVTINASSS RSGLDDINPT VLLKERSTEL

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.