Thermo Fisher Scientific ATP Citrate Lyase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_0010872) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP Citrate Lyase,uniProtId:P53396-1,ncbiNodeId:9606,antigenRange:752-1101,antigenLength:1101,antigenImageFileName:PA5-90320_ATP_Citrate_Lyase_P53396-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90320_ATP_Citrate_Lyase_P53396-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.82 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806026

Product Specific Information

Immunogen sequence: FSSEVQFGHA GACANQASET AVAKNQALKE AGVFVPRSFD ELGEIIQSVY EDLVANGVIV PAQEVPPPTV PMDYSWAREL GLIRKPASFM TSICDERGQE LIYAGMPITE VFKEEMGIGG VLGLLWFQKR LPKYSCQFIE MCLMVTADHG PAVSGAHNTI ICARAGKDLV SSLTSGLLTI GDRFGGALDA AAKMFSKAFD SGIIPMEFVN KMKKEGKLIM GIGHRVKSIN NPDMRVQILK DYVRQHFPAT PLLDYALEVE KITTSKKPNL ILNVDGLIGV AFVDMLRNCG SFTREEADEY IDIGALNGIF VLGRSMGFIG HYLDQKRLKQ GLYRHPWDDI SYVLPEHMSM; Positive Samples: HepG2, HeLa, Raji, NIH/3T3, Mouse brain, Mouse heart, Mouse kidney, Rat brain; Cellular Location: Cytoplasm

Target Information

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.