Thermo Fisher Scientific Luciferase Polyclonal Antibody
Luciferase 단백질 검출용 염소 유래 폴리클로날 항체. Western blot, ELISA, IP 등 다양한 응용 가능. Firefly luciferase에 특이적으로 반응하며 Renilla luciferase에는 반응하지 않음. 장기 보관 시 -20°C에서 냉동 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Luciferase |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Luciferase (Firefly) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 82 mg/mL |
| Storage Buffer | 0.02 M potassium phosphate/whole serum, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Luciferase (Firefly). No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Target Information
Luciferase produces green light with a wavelength of 562 nm.
The enzyme catalyzes the reaction:
D-firefly luciferin + O₂ + ATP → firefly oxyluciferin + CO₂ + AMP + diphosphate + light
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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