Thermo Fisher Scientific alpha-1-Anti-Trypsin Polyclonal Antibody
Human alpha-1-Anti-Trypsin을 인식하는 Goat Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 면역실험에 사용 가능. Lyophilized 형태로 안정적이며 장기 보관 시 -20°C에서 보관 권장. 연구용으로만 사용.
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- 100-101-147
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Thermo Fisher Scientific alpha-1-Anti-Trypsin Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | a1-Anti-Trypsin (Human Plasma) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 90 mg/mL |
| Storage Buffer | 0.02M potassium phosphate / whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified a1-Anti-Trypsin (Human Plasma).
- Cross-reactivity against a1-Anti-Trypsin from other sources is unknown.
Target Information
Alpha-1-AT is synthesized in the liver and acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung.
In the absence of alpha-1-AT, these enzymes are not inhibited and may digest pulmonary parenchyma.
Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn.
Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation.
Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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