Thermo Fisher Scientific Ribonuclease A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:500-1:5,000

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-RIBONUCLEASE A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.

Target Information

Endonuclease that catalyzes the cleavage of RNA on the 3` side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.