Thermo Fisher Scientific Human THRB, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

• Ligand Binding Assay (LBA)

Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Thyroid Receptor beta, Ligand Binding Domain (THRB LBD) is a 57.40 kDa recombinant human protein (amino acids 202–461) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. THRB LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Protein Form: Recombinant, Ligand Binding Domain

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, regulate metabolic processes, growth, and development in many tissues by controlling the expression of genes for growth hormone, malic enzyme, and several hepatic proteins.

There are two forms of TR: TR alpha (chromosome 17) and TR beta (chromosome 3). Each has two isoforms — TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2. TR alpha-1 and TRv alpha-2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and acts as a strong negative regulator of functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.