Thermo Fisher Scientific Citrulline Monoclonal Antibody (6C2.1), PE
Citrulline 단백질 잔기를 검출하는 PE-conjugated mouse monoclonal 항체. WB, IHC, ICC, ELISA에 사용 가능. 단백질 시트룰린화 연구에 적합하며, 565/576 nm 파장에서 형광 검출. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C2.1 |
| Immunogen | Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932214 |
Additional Formats
- Unconjugated (MA5-27574)
- APC (MA5-45757)
- FITC (MA5-45758)
- PerCP (MA5-45759)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45760 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for citrulline residues on modified proteins. Tested using citrulline-conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid not incorporated into proteins during synthesis but generated within proteins by deimination of arginine. It occurs naturally in proteins such as Myelin Basic Protein (MBP), while others, such as fibrin, become citrullinated during inflammation or apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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