Thermo Fisher Scientific Acrolein Monoclonal Antibody (2H2), APC
Acrolein 변형 단백질을 특이적으로 검출하는 마우스 단클론 항체(APC 결합형). Western blot, ICC/IF, Flow cytometry, ELISA에 적합. 화학적 반응성 단백질 연구용. 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H2 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932215 |
Available Formats
- Unconjugated
- FITC
- PE
- PerCP
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45761 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein-modified proteins. Does not detect free Acrolein.
No cross-reactivity with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may also form from the breakdown of certain pollutants in outdoor air or from the burning of organic matter such as tobacco, gasoline, or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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