Thermo Fisher Scientific ATG4D Polyclonal Antibody
ATG4D 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합. 인간 및 생쥐 시료 반응성. 친화 크로마토그래피로 정제된 액상 항체이며, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ATG4D (Accession Q86TL0), corresponding to amino acid residues Y425–L474. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2854318 |
Product Specific Information
This antibody detects endogenous levels of total ATG4D.
Target Information
Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein localized in the cytoplasm and a member of the C-54 family of cysteine proteases. It is predominantly expressed in skeletal muscle and testis. Atg4D functions as a cysteine protease required for autophagy, specifically cleaving the C-terminal region of target proteins to enable their binding to autophagosomes. Its enzymatic activity is inhibited by N-ethylmaleimide, a thiol-reactive compound that modifies cysteine residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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