Thermo Fisher Scientific RPA4 Polyclonal Antibody
RPA4 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 면역형광(ICC/IF)에 적합합니다. 인간 시료에 반응하며, 친화 크로마토그래피로 정제된 액상 형태입니다. DNA 복제 및 수선 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RPA4 (Accession Q13156), corresponding to amino acid residues V162–C212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852533 |
Product Specific Information
Antibody detects endogenous levels of total RPA30.
Target Information
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages, and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB consisting of three subunits that play an important role in DNA replication, recombination, and repair. RPA is one of the major damage-recognition structures involved in the early stage of nucleotide excision repair and may function in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5′ side of a ssDNA substrate and then extends in the 3′ direction to create a stably bound hRPA. Widely expressed at low to intermediate levels, RPA 34 kDa subunit, also known as RPA4 (replication factor A protein 4), contains 261 amino acids, localizes to the nucleus, and is preferentially expressed in mucosa of colon and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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