Thermo Fisher Scientific POLR3D Polyclonal Antibody
POLR3D 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등에 사용 가능. Human RNA polymerase III subunit D의 175–225번 영역을 면역원으로 제작. 액상 형태, 4°C 보관, 연구용 전용 제품.
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Thermo Fisher Scientific POLR3D Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 and 225 of Human DNA-directed RNA polymerase III subunit D (UniProt ID: P05423-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
This gene encodes a protein that interacts with the N-terminal region of BRCA1. It shares homology with the two most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in proteins regulating cell growth, including tumor suppressor genes and protooncogenes.
This protein also contains three tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, suggesting that a stable complex between these proteins is essential for BRCA1 tumor suppression.
This protein may be the target of oncogenic mutations in breast or ovarian cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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