Thermo Fisher Scientific LDHA Polyclonal Antibody
LDHA 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF 등에 사용 가능. 인간, 생쥐, 랫드 등 다양한 종에 반응하며, 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 10 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | — |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | — |
| RNA Immunoprecipitation (RIP) | — | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 133–332 of Human Lactate Dehydrogenase A (LDHA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.37 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544882 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, MCF-7, MDA-MB-231, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
- Predicted reactivity: Mouse (92%), Rat (91%), Xenopus laevis (80%), Pig (90%), Rabbit (91%), Chicken (80%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (91%).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity. There are five LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure. LDHA is hypoxia-inducible and regulated by HIF1α, while LDHB is upregulated in many cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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