Thermo Fisher Scientific eIF4E2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human eIF4E2, validated for WB, IHC, ICC/IF, and ELISA. Recognizes 7-methylguanosine mRNA cap and functions as a translation initiation repressor. Supplied as liquid form, affinity purified, for research use only.
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Thermo Fisher Scientific eIF4E2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–240 of human EIF4E2 (NP_0048371) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901627 |
Product Specific Information
- Positive Samples: BT-474, HL-60, Mouse kidney
- Immunogen sequence:
MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTERDKNQS SSKRKAVVPG PAEHPLQYNY TFWYSRRTPG RPTSSQSYEQ NIKQIGTFAS VEQFWRFYSH MVRPGDLTGH SDFHLFKEGI KPMWEDDANK NGGKWIIRLR KGLASRCWEN LILAMLGEQF MVGEEICGAV VSVRFQEDII SIWNKTASDQ ATTARIRDTL RRVLNLPPNT IMEYKTHTDS IKMPGRLGPQ RLLFQNLWKP
Target Information
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in translation initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). Unlike EIF4E, it does not bind eIF4G (EIF4G1, EIF4G2, or EIF4G3), suggesting competitive inhibition of eIF4F complex formation at the cap. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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