Thermo Fisher Scientific ATP6AP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP6AP1,uniProtId:Q15904-1,ncbiNodeId:9606,antigenRange:90-390,antigenLength:470,antigenImageFileName:PA5-120143_ATP6AP1_Q15904-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120143_ATP6AP1_Q15904-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.36 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913715

Product Specific Information

Positive test controls include: THP-1, OVCAR3, PC-3, Mouse brain. The target is usually found in the following locations: Single-pass membrane protein, Vacuole membrane.

Immunogen sequence: LLFLQDKLSI EDFTAYGGVF GNKQDSAFSN LENALDLAPS SLVLPAVDWY AVSTLTTYLQ EKLGASPLHV DLATLRELKL NASLPALLLI RLPYTASSGL MAPREVLTGN DEVIGQVLST LKSEDVPYTA ALTAVRPSRV ARDVAVVAGG LGRQLLQKQP VSPVIHPPVS YNDTAPRILF WAQNFSVAYK DQWEDLTPLT FGVQELNLTG SFWNDSFARL SLTYERLFGT TVTFKFILAN RLYPVSARHW FTMERLEVHS NGSVAYFNAS QVTGPSIYSF HCEYVSSLSK KGSLLVARTQ P

Target Information

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, compose the V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c` and c``) make up the V0 domain. ATP6AP1 (ATPase, H+ transporting, lysosomal accessory protein 1), also known as 16A, CF2, Ac45, XAP3, ATP6S1, VATPS1 (vacuolar ATP synthase S1 accessory protein) or ATP6IP1, is a type I transmembrane, V-ATPase accessory protein that is predominantly expressed in endocrine and neuronal cells. ATP6AP1 is responsible for targeting the V-ATPase enzyme to specialized complex vacuolar systems. Via its cytoplasmic tail, ATP6AP1 interacts with subunits of the V0 domain. The disruption of this interaction in osteoclasts results in impaired bone resorption, suggesting an important role for ATP6AP1 in proper osteoclastic bone resorption.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.