Thermo Fisher Scientific Mouse GM-CSF Recombinant Protein

Applications and Tested Dilution

Product Specifications

Product Specific Information

Carrier-Free

• Reconstitution:
  Centrifuge the vial briefly before opening to bring the contents to the bottom.
  Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1–1.0 mg/mL.
  Apportion the reconstituted protein into working aliquots and store at ≤ -20°C.
  Make any further dilutions in low endotoxin medium or buffered solution containing a carrier protein (e.g., heat-inactivated FCS or tissue culture grade BSA).

• Storage:
  Store the lyophilized protein at 2–8°C, preferably desiccated.
  Upon reconstitution, apportion into working aliquots and store at ≤ -20°C.
  Avoid repeated freeze-thaw cycles.

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at chromosome region 5q31, associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated cytokine levels, including those produced by this gene, have been observed in SARS-CoV-2–infected patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.