Thermo Fisher Scientific MiTF Recombinant Rabbit Monoclonal Antibody (ARC0660)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0660

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 387-509 of human MITF (O75030) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MiTF,uniProtId:O75030-1,ncbiNodeId:9606,antigenRange:387-509,antigenLength:526,antigenImageFileName:MA5-35146_MiTF_O75030-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35146_MiTF_O75030-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849051

Product Specific Information

Immunogen sequence: HLLLRIQELE MQARAHGLSL IPSTGLCSPD LVNRIIKQEP VLENCSQDLL QHHADLTCTT TLDLTDGTIT FNNNLGTGTE ANQAYSVPTK MGSKLEDILM DDTLSPVGVT DPLLSSVSPG ASK

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.