Thermo Fisher Scientific MBP Polyclonal Antibody
Rabbit polyclonal antibody against human MBP for WB and IHC applications. Recognizes MBP with high specificity. Suitable for research use only. Lyophilized form, reconstitute in sterile water. Stable under recommended storage conditions.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human MBP conjugated to blue carrier protein was used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: MBP.
Target Information
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also present in the bone marrow and immune system. These arise from the long MBP gene (also called Golli-MBP) that contains 3 additional upstream exons.
Alternative splicing from the Golli and MBP transcription start sites produces 2 sets of MBP-related transcripts and gene products. Golli mRNAs encode hybrid proteins with N-terminal Golli sequence linked to MBP sequence, while MBP-only transcripts produce the well-characterized myelin basic proteins.
This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and important for gene regulation and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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