Thermo Fisher Scientific HSPB8 Monoclonal Antibody (3C12-H11), FITC
HSPB8 단백질을 인식하는 FITC 결합 단클론 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA 등에 사용 가능하며, 498/517 nm 형광 특성을 가집니다. 단백질 G 정제, 액상 형태, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C12-H11 |
| Immunogen | His-tagged human recombinant HSP22 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 581 mM DMSO, 123 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931708 |
Additional Formats
- Unconjugated (MA5-27706)
- APC (MA5-45253)
- PE (MA5-45256)
- PerCP (MA5-45255)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45254 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 22 kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric, and tetrameric forms in WB.
Does not cross-react with alpha crystallin.
Target Information
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conserved alpha-crystallin domain at the C-terminal part of the molecule.
Its expression is induced by estrogen in estrogen receptor-positive breast cancer cells, and it functions as a chaperone in association with Bag3, a stimulator of macroautophagy.
This gene is involved in regulation of cell proliferation, apoptosis, and carcinogenesis. Mutations have been associated with neuromuscular diseases including Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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