Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:10,000

-

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 8 publications 8 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Luminex (LUM)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

LN3

Immunogen

Activated human peripheral blood mononuclear cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR,uniProtId:P01903-1,ncbiNodeId:9606,antigenRange:1-254,antigenLength:254,antigenImageFileName:MA5-11966_HLA-DR_P01903-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_P01903-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:HLA-DR,uniProtId:P79483-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-11966_HLA-DR_P79483-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_P79483-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:HLA-DR,uniProtId:P01911-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-11966_HLA-DR_P01911-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_P01911-1_House_mouse_PDP.jpeg,sortOrder:3},{targetFamily:HLA-DR,uniProtId:P04233-1,ncbiNodeId:9606,antigenRange:1-296,antigenLength:296,antigenImageFileName:MA5-11966_HLA-DR_P04233-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_P04233-1_House_mouse_PDP.jpeg,sortOrder:4},{targetFamily:HLA-DR,uniProtId:P13762-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-11966_HLA-DR_P13762-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_P13762-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:HLA-DR,uniProtId:Q30154-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-11966_HLA-DR_Q30154-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11966_HLA-DR_Q30154-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979984

Product Specific Information

MA5-11966 targets Human MHC II (HLA-DR) in FACS, WB and IHC (F) applications and shows reactivity with Human samples. This antibody does not react with mouse tissue in Western blot applications.

The MA5-11966 immunogen is activated human peripheral blood mononuclear cells.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.