Thermo Fisher Scientific SMO Polyclonal Antibody
SMO 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼(0.1% sodium azide 포함) 중 1 mg/mL 농도로 제공. 다양한 종에 반응하며 Hedgehog 신호전달 연구에 적합.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC(P)) | 10 µg/mL | Heat-induced antigen retrieval in pH 6.0 citrate buffer recommended |
| Immunocytochemistry (ICC/IF) | Assay-dependent | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Horse, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551570 |
Product Specific Information
Percent identity with other species by BLAST analysis:
Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 amino acids. It contains seven transmembrane domains, four glycosylation sites, and a putative extracellular amino terminus.
Smoothened is activated by β-Arrestin 2 and GRK2, acting as a receptor for Sonic hedgehog (Shh), and serves as a positive regulator of the hedgehog signaling pathway. Together with patched protein (PTCH), it regulates vertebrate developmental processes, cellular proliferation, and differentiation by activating transcription factor Gli.
Mutations in Smoothened are associated with sporadic basal cell carcinoma of the skin. It is widely expressed in heart and brain tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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