Thermo Fisher Scientific SMO Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Bat, Bovine, Dog, Horse, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMO,uniProtId:Q99835-1,ncbiNodeId:9606,antigenRange:1,antigenLength:787,antigenImageFileName:PA5-34217_SMO_Q99835-1_Rabbit.svg,antigenImageFileNamePDP:PA5-34217_SMO_Q99835-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2551570

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).

For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.