Thermo Fisher Scientific ACBD3 Polyclonal Antibody
인간 ACBD3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ACBD3. Recombinant protein control fragment (Product #RP-90777). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2637587 |
Product Specific Information
Immunogen sequence:
QYPGNYEQQQ ILIRQLQEQH YQQYMQQLYQ VQLAQQQAAL QKQQEVVVAG SSLPTSSKVN ATVPSNMMSV NGQAKTHTDS SEKELEPEAA EEALENGPKE SLPVIAAPSM WTRPQIKDFK EKIQQDADSV IT
Highest antigen sequence identity to the following orthologs:
- Mouse: 89%
- Rat: 86%
Target Information
GOLPH1, also known as GCP60, was initially identified as a Golgi protein that interacts with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure.
It also interacts with other Golgi proteins such as Golgin-160, which can be cleaved by caspases-2, -3, and -7, leading to nuclear localization of Golgin-160. The interaction between GOLPH1 and Golgin-160 fragments is stronger than with the intact form, regulated by the oxidation state of Cys-463 within GOLPH1.
Additionally, GOLPH1 interacts with Numb, a cytosolic signaling protein mediating asymmetric cell division in neural progenitor cells, suggesting Golgi fragmentation and reconstitution during the cell cycle may regulate Numb signaling and couple cell fate with cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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