Thermo Fisher Scientific USP15 Monoclonal Antibody (1C10)
USP15 단백질을 인식하는 마우스 단클론 항체(클론 1C10)로, Western blot, ICC/IF, ELISA에 적합. 인간 단백질 반응성. 비결합형 액상 포맷, PBS(pH 7.4) 버퍼, 보존제 없음. -20°C 보관, 동결/해동 반복 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C10 |
| Immunogen | USP15 (AAH20688, 1 a.a. ~ 235 a.a.) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein Sequence:
MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFS IPDEKETRLW NKYMSNTFEP LNKPDSTIQD AGLYQGQVLV IEQKNEDGTW PRGPSTPKKP LEQSC
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.
Deubiquitination, catalyzed by deubiquitinating enzymes, provides an additional level of ubiquitin regulation. These enzymes fall into four families:
- Ubiquitin C-terminal hydrolases
- Ubiquitin-specific processing proteases (USPs)
- OTU-domain ubiquitin-aldehyde-binding proteins
- Jab1/Pad1/MPN-domain-containing metallo-enzymes
Among these, USPs are the most widespread and conserved across evolution. They release ubiquitin from conjugated proteins and share catalytic domains containing conserved Cys and His boxes, with variable terminal extensions involved in substrate recognition, localization, and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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