Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ELISA, IP 등 다양한 실험에 사용 가능. 고순도 친화 크로마토그래피 정제, 안정적인 액상 형태, 세포주기 조절 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 125–165 on E2F1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) containing 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interaction with DP transcription factors)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain embedded within the transactivation domain
E2F1, along with E2F2 and E2F3, possesses an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner, mediating both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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